CYTOKINE secretion - assay or PCR?

Dr Robin Barclay PhD 100066.300 at CompuServe.COM
Mon Feb 12 07:43:32 EST 1996


The cheapest cytokine assays are bioassays - there are a number 
of cell lines which (i) need a particular cytokine or (ii) are 
sensitive to a particular cytokine.  If you have the time and are 
not "rolling in loot" as someone else put it - bioassay may be 
the optimal method.  Out of interest - how will you know which 
cells secreted the cytokines - are you starting with purified 
populations?



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