M_Doherty at NIH.gov
Thu Feb 15 14:07:06 EST 1996
In article <4ft7fg$h3o at netnews.upenn.edu>, David Peritt
<Peritt at mail.med.upenn.edu> wrote:
> In article <4fso3c$hvl at hades.rz.uni-sb.de> Uta Burgard-Wollschlaeger,
> utbu0000 at stud.uni-sb.de writes:
> >Who is working with flow cytometry to detect intracellular cytokines ?
> What are
> >your experiences and what do you use: testkit or protocols ?
> >Is there anyone working with athletes or on the field of sportsmedicine
> with this ?
> >Thanks for your help.
> Pharmingen has a whole series of mouse and human reagents for this
> purpose and will send their technique along with it...... It is easy and
> quite nice if you are doing multicolour testing ie. surface along with
> intracellular. I have used the IFNg human and am quite happy with it...
We are using the Caltag kits for the same purpose and they work well.
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