Advise needed regarding MLR assay

Bob Scibienski rjscibienski at
Wed Feb 14 20:38:56 EST 1996

	I suggest that you dissolve them in a low MW ether or ester,
aliquot the solution into your culture vessels and then let
the solvent evaporate, leaving behind a coating of your
compound.  Then add a macrophage enriched population for 24
hr. or so prior to adding your responder cells.  Some of the
compound should partition into the aqueous phase, but I
would also expect adhering macrophages to scavenge some of
it from the surfaces they are adhering to.

Good luck!

Bob Scibienski

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