Advise needed regarding MLR assay

Nicole at cfi.unsw.edu.au Nicole at cfi.unsw.edu.au
Fri Feb 16 02:25:12 EST 1996


"Dr. Takashi Satoh" <tak> wrote:
>We are working on screening some organic molecules using the in vitro MLR
>(mixed lymphocytes reaction) assay. But we dont't have much experience dealing
>with organic compounds. Can anybody suggest an ideal solvent(s) to dissolve
>these compounds and use them on MLR? Your advise will be greatly appreciated.

I have used DMSO and ethanol in the past.  These are both toxic to cells
in high concentrations but if the compound you are testing is dissolved
in them they can then be diluted in culture medium and added to your
MLR. 




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