Thymocyte-Conditioned Medium...

Dave Sinasac sinasa2 at server.uwindsor.ca
Thu Feb 22 15:53:18 EST 1996


Dear Group,


	We normally prepare thymocyte-conditioned medium (for in vitro 
sensitization of B lymphocytes to produce monoclonal antibodies) by 
incubating 12 to 14 day old mice thymus in DMEM.  This is to produce a 
medium containing a mixture of interleukins which are conducive for B 
cell growth.  I was wondering if anyone who does similar work out there 
has looked into adding a recombinant mixture of ILs and other growth 
factors as an alternative method to this.

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|   Dave Sinasac                             sinasa2 at server.uwindsor.ca   |
|   Department of Biological Sciences        University of Windsor        |
|                            IRC nickname:Rubisco                         |
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