Jurkat Cells and TNF Receptors?

[Lisa Sedger]

I am trying to express a virus proteins from Jurkat cells, using a
constitutive eukaryotic expression vector. I now have stably transfected
cell lines, and am currently characterising them. However, it seems that
Jurkat cells express a number of secreted cellular proteins. Does anyone
know whether Jurkat T cells express TNF receptors? In particular I am
interested in finding out whether they secrete soluble TNFR's - TNFR1 or
TNFR2?

Thanks,
Lisa


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Lisa Sedger
Department of Biochemistry.
University of Alberta.
Edmonton. CANADA.
E-mail: Lisa_Sedger at darwin.biochem.ualberta.ca
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