Cell separations

Corey Burke (BIO) cburke at chuma.cas.usf.edu
Thu Feb 29 11:17:00 EST 1996

On 27 Feb 1996, Marci Chan wrote:

> I would like to separate about 200 million tonsil B cells into different Ig
> subsets using either a panning procedure or magnetic cell separation
> procedure.  Has anyone had experience separating this large a number of
> cells?  My impression is that using the magnetic  cell separation procedure
> would be very costly.
> Thanks!
>How about FACS, Fluorescence activated cell sorter. The equiptment is 
expencive, but the process is not.  > 

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