Hybridomas

Richard C. Harmon cytact at netcom.com
Mon Jan 1 14:20:18 EST 1996


MPbamberg (mpbamberg at aol.com) wrote:
: I have been attempting to grow hybridomas in mice. With the line we are
: using we just get solid tumors, no ascities. Does anyone have any
: suggestions. Thank You

You need to give more information regarding your current procedures.  I
found that setting aside some mice which had been pristane-primed for
longer periods was helpful on occasion.  Also, are we talking an all BALB
combination?  That is, are their any MHC issues to deal with?  If so, a 
little immunosuppression (irradiation) may be in order.

Rick

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