Redundancy in kinased protein detection

Giorgio Spagnol spagnol at galactica.it
Tue Jan 2 14:34:43 EST 1996


Dear fellow researchers,
I need to detect phosphotyrosinated proteins by western blotting, after 
receptor crosslinking of cells in culture.
The only one protocol I have advice immunoprecipitation of cells lysate 
with mAb G410, followed by blotting, using the same mAB as primary 
antibody.
Why not dyalise and concentrate the whole cell lysate, load it in the 
gel and immunoblot DIRECTLY, omitting the immunoprecipitation step?
Any help would be greatly appreciated.

Giorgio Spagnol, MD
Institute of Neurology,
Statal University, Milan,
Via F. Sforza 35,  20122 Milan, Italy.

Phone: 02-55190390 W, 02-6570326 H
FAX:    02- 55190392
E-MAIL: spagnol at galactica.it







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