Dear fellow researchers,
I need to detect phosphotyrosinated proteins by western blotting, after
receptor crosslinking of cells in culture.
The only one protocol I have advice immunoprecipitation of cells lysate
with mAb G410, followed by blotting, using the same mAB as primary
antibody.
Why not dyalise and concentrate the whole cell lysate, load it in the
gel and immunoblot DIRECTLY, omitting the immunoprecipitation step?
Any help would be greatly appreciated.
Giorgio Spagnol, MD
Institute of Neurology,
Statal University, Milan,
Via F. Sforza 35, 20122 Milan, Italy.
Phone: 02-55190390 W, 02-6570326 H
FAX: 02- 55190392
E-MAIL: spagnol at galactica.it
