>In <4c6u3u$9mv at server-b.cs.interbusiness.it> Giorgio Spagnol
><spagnol at galactica.it> writes:
>>>>Dear fellow researchers,
>>I would be bery grateful if anyone could point to me an article
>>illustrating the PRINCIPLES of the immuneprecipitation reaction. It
>was
>>to date impossible to me NOT to find a protocol (there are at least
>one
>>for any immunology lab manual, and in the same book, without any
>reasons
>>the protocols may vary in two different chapters) but to find
>explained
>>WHY you should use a particular buffer,WHAT the advantages or
>>disadvantages of using protein A coupled to agarose instead of Ab
>linked
>>to sepharose as precipitin etc, etc, etc.
>>Thank you very much for any help.
>>Giorgio Spagnol
>>>>>Giorgio,
>>Pharmacia the giant Swedish company that produces sepharose, sephedex,
>sephacryl and all, also sells a series of booklets (about $15 each)
>explaining the principles of various Pharmacia products. I remember
>one booklet on Sephadex and molecular sieving, another on ion exchange
>chromatography and so on. They give you practical hints on how their
>various products are used as well. I don't have any experience using
>affinity purification columns so I can't be more specific. If your
>company orders directly from Pharmacia, getting these booklets are
>simple. Or if you purchase their products indirectly from another
>distributor - say perhaps Sigma Chemical which everyone orders from
>one time or another, check with Sigma directly about availability.
>>Dana
Dana just reminded me that Calbiochem also publishes a book on their fixed
StaphA and its application. Best of all, its free.
Brett Lindenbach
Program in Immunology
Washington University - St Louis
brett at borcim.wustl.edu
"I own my own pet virus. I get to pet and name her." - Cobain
