Problems with Murine IL-2 ELISA

Martin Burkhardt mburkhar at ix.netcom.com
Fri Jan 5 21:03:43 EST 1996


Dear George,

   We are currently quantitating IL-2 in supernatants from in vitro 

cultures of the DO11.10 and GD12 T-cell lines using the Pharmingen IL-2 

ELISA. The only problems we've had have been when we used anything but PBS 

10% FBS to dilute standards and samples. Without the 10% FBS we get a

dampening of signal and poor linearity of dilution.



                                         Marty Burkhardt

                                         American Dade

                                         mburkhar at ix.netcom.com




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