Dear Michael,
I'm not sure from your letter whether or not you already have your CD4 T
cell clones or whether you need info on how to generate them. Either
way, the subject is rather like gardening and everybody has their own
methods. You will need to get hold of some Human AB serum (from a local
Blood Bank) to start with. You will also need very clean Mycoplasma free
culture equipment (pref. only used for T cell culture). We generally
stimulate T cells weekly but this depends on the specificity of the
cells you wish to grow. Please contact me with more specific details of
what you intend to do and I'm sure I can help.
Cheers Russ
RRRP87C at prodigy.com (Matthew Dubeck) wrote:
>I would like to grow human T4 helper cells in some sort of growth medium,
>but need some direction as to where I should start. Can anyone send me a
>reference to a procedure for maintaining a human T4 helper culture?
>Thanks.
>> Matthew Dubeck
>RRRP87C at prodigy.com>
