xiaoke zhang <xzhang at kumc.edu> wrote:
>Hello, everyone:
I am working with .. mouse peritoneal macrophages. I
>found that, when I used the TRI-zol reagent (GIBCO.BRL) to extract RNA, I
>could not have the RNA pellet dissolved in water...Does anyone have
>other ideas to extract RNA from this type of cells?
We ran into the same problem with another type of cell. Technical support for Trizol
suggested that we dissolve the RNA in TE buffer. Have had no problems since.
PC Grimm
Pediatric Nephrology/Transplant Immunology
U Manitoba
Canada
