We are working in a kinetic assay (in microtiter plates) designed by Virella
et al, it works pretty neat and is been tested by us on monocytes and PMN`s
all you will need is an ELISA reader able to perform kinetic curves.
Reference: Virella et al: J.Clin. Lab. Anal. 1990, 4:86-89.
Good Luck
Siham
On 6 Dec 1995, Meike Mueller wrote:
> We are investigating the O2-radical-production of PMNs and
> macrophages. Does anyone know a method to measure O2-radical production
> with the NBT-reduction assay in microtiterplates?
> Thanks a lot
> Meike Mueller Fraunhofer ITA Hannover FRG
> email: MMueller at ita.fhg.de>>>
