Hi! I am interested in growing T-cells
over extended periods. My boss said that it is
usually done by taking isolated T-cells, stimulating
them with PHA over-night, washing out the PHA and
culturing them with IL-2. Repeating the PHA stim-
ulation periodically. Unfortunately, he doesn't
have the original reference. I haven't been able
to find it either.
If anyone knows the specifics of
this and has the reference, I would be very
appreciative.
Richard Palmer
Palmer at MSVAX.MSSM.EDU
Box 1198
Mt. Sinai Med. Center
One Gustav Levy Place
New York, NY 10029
