Help:Induction of CTL

Dave Peritt peritt at
Mon Jul 8 07:25:25 EST 1996

In article <01BB6992.1833A4C0 at> Tomomi
Teraoka, t_teraoka at writes:
>Is there anything wrong with my method?

You do not have any help (ie CD4 stimulation).  Spleen cells work because
dentritics etc. stimulate CD4 which help CD8 via cytokines primarily. 
You may try adding allo CD4 stimulators (and later sort) or try adding
ConA stimulated sup to your hep/CTL culture.

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