Some manufacturers even recommentd freezing the product for prolonged
storage. The major problem are freeze saw cycles thus stay away from
frost-free freezers. Also some antibodies become inactivated at given
temperatures between 0 and -20oC, thus if possible put alliquots at
-80oC.
GERHARD.NEBE-VON-CARON at UNILEVER.COM
> Subject: DON'T FREEZE ABS
>> Date: 11 Jul 1996 17:57:26 GMT
>> In article <4s3aog$g8m at unix13.u-3mrs.fr> Serge Birman,
>birman at lnf.cnrs-mrs.fr writes:
> >I just purchased a monoclonal antibody. This Mab is shipped
> lyophilized
> >and the company recommends to dissolve it in water and keep in the
> >fridge. However, for longer storage, I would like to store it in
> >aliquots in the freezer. Is that possible without losing too much
> >activity? Which buffer should I use? With or without glycerol? I was
> >wondering whether glycerol can be harmful to some antibody.
>> this question is raised periodically on this board. mAbs are very
> stable
> in PBS at 4C if stored at 1mg/ml or in a carrier solution of BSA or
> glycerol. We store both conjugated and unconjugated Abs for years
> under
> these conditions. Freezing is actually harmful esp. for conjugated
> Abs.....
>
