b2d6 at musicb.mcgill.ca wrote:
>> I would appreciate any references and/or protocols on purifying
> B-lymphoblasts from a mixed monocyte population (like Ficoll-Hypaque
> purified WBCs for example).
>...after retrieving the lymphocyte fraction from a Ficoll gradient, run
everything over a nylon wool column: I know that the B-cells and
monocytes are nylon wool adherent, while the T- and NK cells will pass
through as nonadherent cells (I think you can find more about this in
Current Protocols in Immunology or something...unfortunately, I don't
have a lot of the details myself).
Hope this helps a bit,
Kiley R. Prilliman