problems with Mab purification

Randy Fischer fischerr at ncifcrf.gov
Wed Jun 5 10:28:27 EST 1996

Mouse IgG2a can be readily purified off Protein A instead of Protein 
G. I suggest you check how you elute the antibody from your column. If 
you use acid elution, be sure to have a neutralization buffer (we use 
TRIS-BASE) in your tube when the eluate comes in-rapid neutralization 
can be critical.

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