Human IL12 ELISA cytokine quantitation
Birgit Sehested & Jens Rainer Hansen
jrhbsh at post4.tele.dk
Wed Jun 5 04:32:28 EST 1996
I want to develop a sandwich ELISA to quantitate the human IL12 cytokine, using a
monoclonal catching antibody and a biotinylated poly- or monoclonal antibody as the
detector antibody. The ELISA should only detect the p70 heterodimer or the p35 protein
(not the biological inactive p40). I do not want to use a commercial kit, only
Do you have any proposals concerning where I can get antibodies which specifically are
directed against the p70 and/or the p35 protein, and an idea of how many mcg/ml the
microtiterplates should be coated with and the amount of detector antibody.
I am new on the Internet, do you know if there are other relevant newsgroups where it is
possible to discuss items like ELISA, cytokines and assay technology.
Jens Rainer Hansen
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