Anti-HLA Class I MAb

David L. Haviland, Ph.D. haviland at KIDS.WUSTL.EDU
Wed Jun 5 22:16:12 EST 1996


On Wed, 5 Jun 1996, Kiley R. Prilliman wrote:

> ...what do you mean by "framework" antibody?  One that specifically 
> binds the heavy chain in a native-molecular state?  I would think it 
> would be easier to use an antibody to the light chain 
> (b2-microglobulin), since there is quite a bit of "species-specificity" 
> with such antibodies.  (For example, the MAb to human b2m, BBM.1, will 
> bind to human and chimpanzee MHC class I but NOT to mouse or even baboon 
> molecules).

Comment (not quite on topic):  By framework, I think he means that the 
MAb (W6/32, for example) recognizes and binds to a (the?) monomorphic 
determinant on Class-I HLA.  That determinant doesn't change in the human 
population and may likely be very conserved among different species.

I know for a fact that W6/32 is a superb positive control when looking at 
COS cells (either 1 or 7's) by flow cytometry.  

David

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