On Wed, 5 Jun 1996, Kiley R. Prilliman wrote:
> ...what do you mean by "framework" antibody? One that specifically
> binds the heavy chain in a native-molecular state? I would think it
> would be easier to use an antibody to the light chain
> (b2-microglobulin), since there is quite a bit of "species-specificity"
> with such antibodies. (For example, the MAb to human b2m, BBM.1, will
> bind to human and chimpanzee MHC class I but NOT to mouse or even baboon
> molecules).
Comment (not quite on topic): By framework, I think he means that the
MAb (W6/32, for example) recognizes and binds to a (the?) monomorphic
determinant on Class-I HLA. That determinant doesn't change in the human
population and may likely be very conserved among different species.
I know for a fact that W6/32 is a superb positive control when looking at
COS cells (either 1 or 7's) by flow cytometry.
David
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