Human IL12 ELISA

Birgit Sehested & Jens Rainer Hansen jrhbsh at post4.tele.dk
Mon Jun 3 13:59:54 EST 1996


Hi ! 

I am sorry the mail concerning the IL2 ELISA was a mistake !!!

I want to develop a human IL12 sandwich ELISA, using a monoclonal catching antibody and 
a biotinylated poly- or monoclonal antibody as the detector antibody. The ELISA should
only detect the p70 heterodimer or the p35 protein (not the biological inactive p40).

I do not want to use a commersial kit, only the antibodies.

Do you have any proposals concerning where I can get the antibodies which specifically 
are directed against the p70 or p35 protein, and an idea of how many mcg/ml the 
microtiter plates should be coated with and the amount of detector antibody.

I am relatively new on the Internet, do you know if there are other relevant newsgroups
to discuss items like ELISA, cytokines and assay technology ?

I would be pleased if somebody could give me some relevant information.


Jens Rainer Hansen



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