I want to develop an sandwich ELISA, using a monoclonal catching antibody and a
biotinylated poly- or monoclonal antibody as the detector antibody. The ELISA should
only detect the p70 heterodimer or the p35 protein (not the biological inactive p40).
I do not want to by a commersial kit, only the antibodies.
Do you have any proposals concerning where I can by antibodies which specifically are
directed against the p70 or p35 protein, and an idea of how mcg/ml the microtiterplates
should be coated with and the amount of detector antibody.
I am relatively new on the Internet, do you know if there are other relevant newsgroups
to discuss items like ELISA, cytokines and assay technology ?
I would be pleased if somebody could give me some relevant information.
Yours
Jens Rainer Hansen
