I want to develop a sandwich ELISA to quantitate the human IL12
cytokine, using a monoclonal catching antibody and a biotinylated poly-
or monoclonal antibody as the detector antibody. The ELISA should only
detect the p70 heterodimer or the p35 protein (not the biological
inactive p40). I do not want to use a commercial kit, only commercial
antibodies.
Do you have any proposals concerning where I can get antibodies which
specifically are directed against the p70 and/or the p35 protein, and an
idea of how many mcg/ml the microtiterplates should be coated with and
the amount of detector antibody.
I am new on the Internet, do you know if there are other relevant
newsgroups where it is possible to discuss items like ELISA, cytokines
and assay technology.
Yours
Jens Rainer Hansen
Research scientist