I want to develop a sandwich ELISA to quantitate the human IL12 cytokine, using a
monoclonal catching antibody and a biotinylated poly- or monoclonal antibody as the
detector antibody. The ELISA should only detect the p70 heterodimer or the p35 protein
(not the biological inactive p40). I do not want to use a commercial kit, only
commercial antibodies.
Do you have any proposals concerning where I can get antibodies which specifically are
directed against the p70 and/or the p35 protein, and an idea of how many mcg/ml the
microtiterplates should be coated with and the amount of detector antibody.
I am new on the Internet, do you know if there are other relevant newsgroups where it is
possible to discuss items like ELISA, cytokines and assay technology.
Yours
Jens Rainer Hansen
