Hi
Wondering if anyone out there has worked out a method to use 10% neutral
bufferred formalin (as the lavage fluid, reverse-flushed through mouse
nose, immediately after death) for cystospin staining to view epithelial
cells, neutros, macs, etc.
My initial attempts have resulted in few cells turning up on the slides
(while parallel PBS lavages work fine).
Please reply to email as well as newsgroup
Any hints, cautions, etc welcomed also.
Thanks,
--
David Parsons, PhD
dparsons at med.unc.eduhttp://www.unc.edu/~snosrap/dwphome.htm
CF/Pulmonary Research & Treatment Center
UNC @ Chapel Hill, NC, USA, 27599