Dear fellow readers of this newsgroup
Is there anyone who has used the Alamar Blue method for measuring the
stimulation of murine spleen cells with a polyclonal stimulator such
as Con A and/or an antigen of interest? In some of the assays we
performed in the wells of microtitre trays the cells in culture alone
changed the colour of the dye. Any advice re a successful protocol
and details of the numbers of cells to use per well, Con A
concentrations and antigen concentrations would be much appreciated.
You may prefer to email your reply direct to me at
igaren at info.curtin.edu.au
Thanks in advance ......
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| Dr Norman Gare Phone: (09) 351 7474
| Senior Lecturer Fax: (09) 351 2342
| Building 308 Room 204 Email: igaren at info.curtin.edu.au| School of Biomedical Sciences
| Division of Health Sciences .
| Curtin University of Technology _--_|\
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