B lymphocytes in mouse spleen

Richard R. Hardy rr_hardy at fccc.edu
Sun Jun 16 16:44:21 EST 1996


In article <31C1571C.5250 at hkstar.com>, chuiyl at hkstar.com wrote:

[what is the percentage of B cells in mouse spleen?]
Our spleen cell suspensions mostly fall in a generous "lymphoid gate" in
terms of forward and side scatter; in BALB/c, about 50% of these cells are
IgM+ (and also CD19+ and B220(6B2)+, for that matter).  This does vary
somewhat with strain, with BALB/c being higher than C57BL/6 (in our
experince).  Most of the other cells are T cells, with about 5% non-T,
non-B.  Of course this is for adult spleen (>2 mos)...  Good luck with
your analyses!
____________________________________________________________________________
Richard R. Hardy                       Member, Institute for Cancer Research           
Fox Chase Cancer Center                Tel:    (215) 728-2463
7701 Burholme Ave.                     FAX:    (215) 728-2412
Philadelphia, PA 19111                 E-MAIL: RR_HARDY at fccc.edu



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