Anti-hamster IgG antibody?

Technical Assistance TechAssist at pierce.geis.com
Mon Jun 17 09:50:32 EST 1996

Shinwa Shibata wrote:
> Most monoclonal antibodies of hamster IgG isotype are from hybridomas established
> by fusing myeloma cells and cells of Armenian hamster.
> Because Armenian hamster and most popular Syrian hamster belong to different
> species each other (Armenian hamster = Cricetulus migratorius, Syrian hamster =
> Mesocricetus auratus), I wonder if I can use usual commercial anti-hamster IgG as
> a 2nd reagent to detect the 1st Ab of Armenian hamster IgG isotype?
> Thank you for your attention and for any comments.
> Shinwa Shibata

Most commercially available anti-hamster antibodies are made to the more 
popular Syrian species. These antibodies will maintain a 70 to 75% 
crossreactivity with  Armenian species. So an anti-Syrian antibody 
can be used to detect antibodies raised in Armenian hamsters.
Tom Brotcke

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