Shinwa Shibata wrote:
>> Most monoclonal antibodies of hamster IgG isotype are from hybridomas established
> by fusing myeloma cells and cells of Armenian hamster.
>> Because Armenian hamster and most popular Syrian hamster belong to different
> species each other (Armenian hamster = Cricetulus migratorius, Syrian hamster =
> Mesocricetus auratus), I wonder if I can use usual commercial anti-hamster IgG as
> a 2nd reagent to detect the 1st Ab of Armenian hamster IgG isotype?
>> Thank you for your attention and for any comments.
>> Shinwa Shibata
Most commercially available anti-hamster antibodies are made to the more
popular Syrian species. These antibodies will maintain a 70 to 75%
crossreactivity with Armenian species. So an anti-Syrian antibody
can be used to detect antibodies raised in Armenian hamsters.
Tom Brotcke
