If you make a single cell suspension from a mouse spleen by
mashing it through a mesh or similar, then about 40% of the cells
you get out will be B cells (positive for surface Ig). See (ahem)
A.H. Fell and P.M. Preston, International Journal for Parasitology
1992, vol. 22 p. 491.
However, I think there will be some cells left in the spleen tissue
you discard, such as dendritic cells, endothelial cells etc which
are not easily dispersed by mechanical means. I do not think anyone
has assessed this, and usually, us immunologists being simple folks,
we chuck them away and regard the lymphocytes as the cells that matter.
I hope this is helpful.
Andy Fell
"He's not the Messiah. He's a very naughty boy."
Chui Yiu-Loon (chuiyl at hkstar.com) wrote:
: I wonder whether anyone would kindly tell me what proportion B cells
: is in adult mouse spleen single cell suspension? This sounds a very
: simple question, but I find it difficult to get a straight answer from
: standard textbooks. In Cellular and Molecular Immunology (2nd edition)
: by Abbas et al., it says B lymphocytes form 40-45% of total
: lymphocytes in the spleen (human or mouse, not specified). But then
: what proportion total lymphocytes make in the entire spleen cell
: population? - No answer on that. In Basic and Clinical Immunology (7th
: edition) by Stites et al., it says B cells make up 40% of lymphocytes
: in spleen marginal zone-white pulp, but again not the information I
: would like to have. Perhaps it is too simple that no one cares any
: more to know about. Thanks for any help in advance.
: Yiu-Loon Chui, Ph.D
: Clinical Immunology Unit
: Chinese Univeristy of Hong Kong
