Utilization of AEC in immunohistochemistry

CW Tuk cw.tuk.cell at med.vu.nl
Wed Jun 19 01:35:54 EST 1996


In article <4q7h8g$gtd at mserv1.dl.ac.uk>, gsabina at pcnet.ro (Gheorghe Sabina) says:
>
>We intend to use the peroxidase substrate 3-amino-9-ethylcarbazole (AEC)
>in an immunohistochemical procedure.
>Unfortunately we have the compound but we haven't the protocol. 
>Any indication concerning the substrate preparation and the conditions
>of reaction would be very helpful to us. 
>If you think that it is not the appropriate newsgroup we shall be grateful
>to everybody who will give us the name of microscopy newsgroup.
>                     
>                     With many thanks in advance
>                                      Sabina Gheorghe
>                                    
>
Hi,

You can make a stock solution of AEC: 0.4 % in dimethylformamide 
(Sigma A-5754).

Incubatie your preparations with a mixture of:

0.5 ml AEC stock
9.5 ml 0.05 M Sodiumacatate pH 5.0
0.1 ml 3% H2O2 (hmmm.... underscript still not possible)

Filter this solution before use.

Embed your preparations in a water containing mediumn like aquamount or
glycerol 50%.

Succes 

Kees



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