Anti-hamster IgG antibody?
abherman at ix.netcom.com
Tue Jun 18 16:58:56 EST 1996
In <31C570B8.55C5 at pierce.geis.com> Technical Assistance
<TechAssist at pierce.geis.com> writes:
>Shinwa Shibata wrote:
>> Most monoclonal antibodies of hamster IgG isotype are from
>> by fusing myeloma cells and cells of Armenian hamster.
>> Because Armenian hamster and most popular Syrian hamster belong to
>> species each other (Armenian hamster = Cricetulus migratorius,
Syrian hamster =
>> Mesocricetus auratus), I wonder if I can use usual commercial
anti-hamster IgG as
>> a 2nd reagent to detect the 1st Ab of Armenian hamster IgG isotype?
>> Thank you for your attention and for any comments.
>> Shinwa Shibata
>Most commercially available anti-hamster antibodies are made to the
>popular Syrian species. These antibodies will maintain a 70 to 75%
>crossreactivity with Armenian species. So an anti-Syrian antibody
>can be used to detect antibodies raised in Armenian hamsters.
Additionally, John Kappler & Pippa Marrack's lab generated an
anti-hamster mAb a couple of years ago, for the purpose of using it as
a secondary antibody with hamster anti-TCR mAbs.
I do not know whether this antibody is commercially available. If
you are interested I could dig up the details of this reagent.
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