In <31C570B8.55C5 at pierce.geis.com> Technical Assistance
<TechAssist at pierce.geis.com> writes:
>>Shinwa Shibata wrote:
>>>> Most monoclonal antibodies of hamster IgG isotype are from
hybridomas established
>> by fusing myeloma cells and cells of Armenian hamster.
>>>> Because Armenian hamster and most popular Syrian hamster belong to
different
>> species each other (Armenian hamster = Cricetulus migratorius,
Syrian hamster =
>> Mesocricetus auratus), I wonder if I can use usual commercial
anti-hamster IgG as
>> a 2nd reagent to detect the 1st Ab of Armenian hamster IgG isotype?
>>>> Thank you for your attention and for any comments.
>>>> Shinwa Shibata
>>Most commercially available anti-hamster antibodies are made to the
more
>popular Syrian species. These antibodies will maintain a 70 to 75%
>crossreactivity with Armenian species. So an anti-Syrian antibody
>can be used to detect antibodies raised in Armenian hamsters.
>Tom Brotcke
Additionally, John Kappler & Pippa Marrack's lab generated an
anti-hamster mAb a couple of years ago, for the purpose of using it as
a secondary antibody with hamster anti-TCR mAbs.
I do not know whether this antibody is commercially available. If
you are interested I could dig up the details of this reagent.
Andrew Herman