VEGF bioassay

C.E. Walters MICCEW at leeds.ac.uk
Fri Jun 21 11:17:22 EST 1996

Does anyone know a reliable method for measuring bioactive VEGF with human 
umbilical vein endothelial cells?
I'd like to know - how many HUVEC to use in 96-well plates, medium, 
incubation time, range of standard VEGF, etc
When I've tried it, I get no measurable response to the VEGF.
Please help!!

Christina Walters
Leeds University

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