I am currently working on an LPS inducible gene which is
superinducible by cycloheximide (CHX), no problem. However, when LPS
and CHX are added together, expression is completely abrogated. I
could understand if the level was either greater than LPS alone or at
least as great as CHX alone. It seems that in the presence of CHX, LPS
acts as an inhibiter instead of an inducer. Has anyone seen this
before or can anyone offer an explanation?
Rob
