Stability of Biotinylated Murine Monoclonals

Richard R. Hardy rr_hardy at fccc.edu
Thu Jun 27 19:14:42 EST 1996

In article <K_Vora-2706961807020001 at manser3.jci.tju.edu>,
K_Vora at lac.jci.tju.edu (Kalpit A. Vora) wrote:

>Dear Fellow immunologists,
>I am having problems in storing biotinylated monoclonal antibodies.They
>are getting inactive upon storage at -20oC. Any suggestion are welcome .
>Please email your responses to kvora at lac.jci.tju.edu
>Thanking you in advance.

Kalpit, don't freeze it!  We store all our labeled antibodies (Fl, Bi, PE,
APC) at 4c in buffer containing 0.1% NaN3.  We've used 10 yr old Mabs that
still "work like new".
Richard R. Hardy          Member, Inst. for Cancer Research           
Fox Chase Cancer Center   Tel:    (215) 728-2463
7701 Burholme Ave.        FAX:    (215) 728-2412
Philadelphia, PA 19111    E-MAIL: RR_HARDY at fccc.edu

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