In article <31D3A375.6FC7 at net.au>, ian at net.au says...
>>I am currently working on an LPS inducible gene which is
>superinducible by cycloheximide (CHX), no problem. However, when LPS
>and CHX are added together, expression is completely abrogated. I
>could understand if the level was either greater than LPS alone or at
>least as great as CHX alone. It seems that in the presence of CHX, LPS
>acts as an inhibiter instead of an inducer. Has anyone seen this
>before or can anyone offer an explanation?
>>Rob
What may be happening, although I don't know the doses that you are
using, is that you are stimulating your cells with LPS, but inhibiting
the cell's response with CHX. This conflict of signals may be driving
your cells to undergo apoptosis, hence giving an apparent inhibition.
