LPS inhibition of CYCLOHEXIMIDE superinduction

Jean-Philip Truman truman at citi2.fr
Fri Jun 28 11:08:02 EST 1996

In article <31D3A375.6FC7 at net.au>, ian at net.au says...
>I am currently working on an LPS inducible gene which is 
>superinducible by cycloheximide (CHX), no problem. However, when LPS 
>and CHX are added together, expression is completely abrogated. I 
>could understand if the level was either greater than LPS alone or at 
>least as great as CHX alone. It seems that in the presence of CHX, LPS 
>acts as an inhibiter instead of an inducer. Has anyone seen this 
>before or can anyone offer an explanation?

What may be happening, although I don't know the doses that you are 
using, is that you are stimulating your cells with LPS, but inhibiting 
the cell's response with CHX. This conflict of signals may be driving 
your cells to undergo apoptosis, hence giving an apparent inhibition.

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