Stability of Biotinylated Murine Monoclonals

WHearl whearl at aol.com
Sun Jun 30 23:09:34 EST 1996

I agree with Dave Peritt - if they are dying at -20, don't freeze them.
(Is your freezer a frost-free type?  If so, be aware they cycle from 0 tp
-20 every few hours to defrost).  As long as they are sterile / preserved,
they should be good for many months.  

If the antibody is denaturing even under mild storage conditions, consider
adding glycerol to 20%, or trehalose to 10%.  These reagents can help
stabilize antibodies.

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