HY antigen and GvH reaxn

Jorg Kirberg kirberg at nki.nl
Fri Mar 1 15:52:47 EST 1996


In article <4h6o27$acq at mserv1.dl.ac.uk>, Neily <NEIL.MABBOTT at bbsrc.ac.uk> wrote:
> I have a question regarding murine HY antigens.
> 
> When reconstituing female SCID mice with male
> bone marrow from the same strain, is a GvH reaction
> likely to occur between the male and female lymphocytes?

Hi Neily,

how shall there be a 'Graft versus Host' reaction ??? Even when there
are T cells contaminating your bone marrow preparation, they would come from
a male mouse, therefore they are tolerant against H-Y (only expressed
in male cells), and in your host, there is no H-Y antigen, as this is a female
mouse.
The other way around ('Host versus Graft reaction'), it should be no
problem either, as your BM recipient is a scid mouse. It will not be able to
react against the male BM; except this mouse is leaky (but see below).

In any case you are on the save side, as (I assume) your scid mice
are H-2<d>. For a H-Y reaction to occur, generally spoken, you require
                                        ^^^^^^^^^^^
presentation on class I and class II MHC antigens. The only class II
MHC molecules that present the H-Y antigen are I-A<b> molecules, which
your mice do not have. In addition, H-Y is only presented on D<b>
and K<k> class I MHC molecules.

I hope that E. Simpson, P. Matzinger, E. Fuchs, C. Melief et al. might
accept this statement ... in any case if you look for literature, those
names are a good starting point. (You also should look for H. von Boehmer,
but he was my supervisor and I am too polite to mention him from the
beginning on).

jorg

kirberg at nki.nl



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