Cloning of CTLs

Singhlab singhlab at
Fri Mar 15 16:21:25 EST 1996

I was wondering if anyone out there would have any information or
suggestions on making CTL clones in vitro using syngeneic target cells
that express a cytosolic protein.  The idea is to transfect a cell
line with the protein so that it is expressed cytosolically and thus
presented through class I to stimulate CD8+ T cells.  Thanks in
advance for any help.

P.S. Please respond to mzechel at

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