In article <scallonb.2.000BB2B3 at voicenet.com>,
David Knight <scallonb at voicenet.com> wrote:
>Hi -
>Does anyone know if there is some inherent problem expressing recombinant
>mouse lymphotoxin alpha (TNF beta) in mammalian cells? I've had lots of
>success with TNF alpha from different species, but can't detect
>expression of mouse LT. Has it been done? Thanks in advance.
>Bernie
There should be no problem, as several investigators have been able to
manipulate this gene (expresion-wise).
For example see:
Browning JL; Dougas I; Ngam-ek A; Bourdon PR; Ehrenfels BN; Miatkowski K;
Zafari M; Yampaglia AM; Lawton P; Meier W; et al.
Characterization of surface lymphotoxin forms. Use of specific monoclonal
antibodies and soluble receptors.
Journal of Immunology, 1995 Jan 1, 154(1):33-46.
(UI: 95088382)
They expressed the gene(s) in CHO cells, and I know that he's done
others, as well. Also, he's a nice guy, and probably won't mind a
call.
I assume (from your previous TNF work) you are aware that unless
you've the beta, the alpha is usually secreted as a trimer.
Also, check the cool article from Matsumoto et. al. from March 1 '96
Science on germinal center formation. You might give David Chaplin's
lab a call for more info:
Center for Immunology, Washington University School of Medicine,
St. Louis, MO 63110, USA.
alias: DAVID-CHAPLIN
name: CHAPLIN, DAVID D
email: CHAPLIN_D at WUMS.WUSTL.EDU
phone: (314) 362-9047
fax: (314) 454-0486
address: Campus Box 8122
department: INTERNAL MEDICINE I & R
title: PROF OF MEDICINE
Good luck!
Aaron
--
"Nothing more is needed to destroy a man, than the conviction that his
life's work is useless." -Antonin Artaud
erawtech at leland.stanford.edu (R. Aaron Warnock)
