In article <4ip4kl$lca at ccshst05.cs.uoguelph.ca>,
Patrick Telmer <ptelmer at uoguelph.ca> wrote:
>I've been setting up MLR's as a part of an undergrad project. I was
>wondering what the role of 2-mercaptoethanol is in the activation of
>cytotoxic T cells in vitro. I can't seem to find any info on this
>particular subject.
Pat,
Can't say I know about CTLs in particular, but 2-ME seems
important for the culture of murine primary cultures (eg as an
additive to cDMEM). Far as I know, the particular part of the serum
that needs reduction isn't clear, but we always but it in as it
doesn't hurt. In moderation, that is... ;-)
In our hands it doesn't make any difference with human cells
in cRPMI...
Good luck,
Aaron
--
"Nothing more is needed to destroy a man, than the conviction that his
life's work is useless." -Antonin Artaud
erawtech at leland.stanford.edu (R. Aaron Warnock)
