MLR - role of 2-ME?

Richard Aaron Warnock erawtech at leland.Stanford.EDU
Wed Mar 20 15:14:52 EST 1996

In article <4ip4kl$lca at ccshst05.cs.uoguelph.ca>,
Patrick Telmer <ptelmer at uoguelph.ca> wrote:
>I've been setting up MLR's as a part of an undergrad project. I was 
>wondering what the role of 2-mercaptoethanol is in the activation of 
>cytotoxic T cells in vitro. I can't seem to find any info on this 
>particular subject.


	Can't say I know about CTLs in particular, but 2-ME seems
important for the culture of murine primary cultures (eg as an
additive to cDMEM). Far as I know, the particular part of the serum
that needs reduction isn't clear, but we always but it in as it
doesn't hurt. In moderation, that is... ;-)

	In our hands it doesn't make any difference with human cells
in cRPMI...

Good luck,


"Nothing more is needed to destroy a man, than the conviction that his
life's work is useless."  -Antonin Artaud

erawtech at leland.stanford.edu (R. Aaron Warnock)

More information about the Immuno mailing list

Send comments to us at biosci-help [At] net.bio.net