Isolation of nulcei

Dr E. Buxbaum EB15 at le.ac.uk
Mon Mar 18 11:51:00 EST 1996

I once tried to break up cells by sonication. This left the nuclei 
morphologically intact, of course I don't know if they will still work 
with your experiment. In any case, you need only a few seconds at low 
power to break the cells open (best to work in an ice/water bath). 

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