Hello,
I am a 3rd year undergraduate at Dalhousie University taking an advanced
immunology course. This course, called "the Granulocytes and the Immune
response" requires me to write a research grant - one that could be
submitted to a granting agency. Sadly, I have no background
in experimental immunology.
My grant involves the isolation of NK cells. The specific protocol that I
am using avoids the use of plastic adherence to remove monocytes since
that involves incubation at 37 C. The authors of a particular paper
(Taub et al. 1995. alpha and beta chemokines induce NK cell migration
and Enhance NK-mediated cytolysis. J. Immuno. 155:3877-3888) claim that
such incubations "appear to inhibit chemokine-specific NK cell
responses...by down-regulation of chemokine receptor expression on
lymphocytes." Have other investigators noticed this effect since it is
the only paper that I have found that mentions this.
Also, is there a good way to label NK cells (i.e.: do they take up 51Cr?)
for a chemotaxis essay?
Answers to these questions can be sent to me at the following email address:
MikeC at is.dal.ca
Thank you for the time.
Mike Charette
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# Oh, #
# the Places You'll Go! #
# You have brains in your head. #
# You have feet in your shoes. #
# You can steer yourself #
# any direction you choose. #
# Dr. Seuss #
# (Theodor S. Geisel, Dartmouth, Class of '25) #
# #
# Michael Charette - Dalhousie University - Halifax, Nova Scotia, Canada #
# MikeC at is.Dal.Ca #
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