NK cell Isolation

Michael Charette mikec at is.dal.ca
Thu Mar 21 00:41:57 EST 1996


Hello,

I am a 3rd year undergraduate at Dalhousie University taking an advanced 
immunology course.  This course, called "the Granulocytes and the Immune 
response" requires me to write a research grant - one that could be 
submitted to a granting agency.  Sadly, I have no background 
in experimental immunology. 

My grant involves the isolation of NK cells.  The specific protocol that I 
am using avoids the use of plastic adherence to remove monocytes since 
that involves incubation at 37 C.  The authors of a particular paper 
(Taub et al. 1995.  alpha and beta chemokines induce NK cell migration 
and Enhance NK-mediated cytolysis.  J. Immuno.  155:3877-3888) claim that 
such incubations "appear to inhibit chemokine-specific NK cell 
responses...by down-regulation of chemokine receptor expression on 
lymphocytes."  Have other investigators noticed this effect since it is 
the only paper that I have found that mentions this.

Also, is there a good way to label NK cells (i.e.: do they take up 51Cr?) 
for a chemotaxis essay?

Answers to these questions can be sent to me at the following email address:

MikeC at is.dal.ca

Thank you for the time.

Mike Charette
 

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