ptelmer at uoguelph.ca (Patrick Telmer) wrote:
>I've been setting up MLR's as a part of an undergrad project. I was
>wondering what the role of 2-mercaptoethanol is in the activation of
>cytotoxic T cells in vitro. I can't seem to find any info on this
>particular subject.
>thanks
>pat
I know that 2-ME at low doses prevents certain disulphide bond formation
that inhibits the function of enzymes, and hence keeps the enzymes in an
active state. I suspect that a similar explanation underlies the
activity of 2-ME in cell culture - ie. prevention of disruptive cysteine
dimerisation.
Mike
