MLR - role of 2-ME?

Michael Daws Michael.Daws at ucmp.umu.se
Fri Mar 22 11:42:02 EST 1996

ptelmer at uoguelph.ca (Patrick Telmer) wrote:
>I've been setting up MLR's as a part of an undergrad project. I was 
>wondering what the role of 2-mercaptoethanol is in the activation of 
>cytotoxic T cells in vitro. I can't seem to find any info on this 
>particular subject.

I know that 2-ME at low doses prevents certain disulphide bond formation 
that inhibits the function of enzymes, and hence keeps the enzymes in an 
active state.  I suspect that a similar explanation underlies the 
activity of 2-ME in cell culture - ie. prevention of disruptive cysteine 


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