Problems of Precipitating Proteins

Stephen McSorley mougneau at
Tue Mar 26 08:05:58 EST 1996

Can anyone suggest a way to keep my protein soluble during purification.  
I'm purifying a protein on Ni NTA resin and end up with the protein in 
8M Urea with imidazole.  After dialysis in PBS the protein precipitates 
such that there is no protein left in solution.  I need the protein 
soluble, anyone got any ideas ?

Stephen McSorley

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