Murine Dendritic Cells

Lee Riley Riley at LOMTM.FCCC.EDU
Wed Mar 27 18:46:20 EST 1996

What's the best way to grow murine dendritic cells.  I've started them with 
GMCSF.  IL-4 seems to inhibit granulocyte outgrowth somewhat.  Others add 

What's the best way to separate the loosely adherent colonies from the single 

Any help would be greatly appreciated.

LB Riley

riley at lomtm.fccc.edu at :

The Fox Chase Cancer Center
7701 Burholme Avenue
Philadelphia, PA 19111
(215) 728-3810

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