What's the best way to grow murine dendritic cells. I've started them with
GMCSF. IL-4 seems to inhibit granulocyte outgrowth somewhat. Others add
indomethicin...
What's the best way to separate the loosely adherent colonies from the single
cells.
Any help would be greatly appreciated.
LB Riley
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riley at lomtm.fccc.edu at :
The Fox Chase Cancer Center
7701 Burholme Avenue
Philadelphia, PA 19111
(215) 728-3810
