I want to investigate the cytokine profiles of several organs (spleen,
liver, lungs) in mice by FACS analysis. Has somebody experience with this kind
of measurements ?
Are other controls than idiotype antibodies necessary, like:
(1) preblocking the antibodies with recombinant cytokines to see if the signal
is specific, or
(2)preblocking of the cytokines with non fluorescent antibodies
Is it possible to see a signal of cytokine producing cells in the complete
mixture of cells in a complete spleen (or worse) in lungs and liver ?
People who have done this kind of experiments could be of great help to me, so
please respond, thanks,