Isolation of nulcei

s1050589 at iplabs.ins.gu.edu.au s1050589 at iplabs.ins.gu.edu.au
Thu Mar 28 22:45:27 EST 1996

In article <4ik49k$924 at ipgate.le.ac.uk> "Dr E. Buxbaum" <EB15 at le.ac.uk> writes:
>From: "Dr E. Buxbaum" <EB15 at le.ac.uk>
>Subject: Re: Isolation of nulcei
>Date: 18 Mar 1996 16:51:00 GMT

>I once tried to break up cells by sonication. This left the nuclei 
>morphologically intact, of course I don't know if they will still work 
>with your experiment. In any case, you need only a few seconds at low 
>power to break the cells open (best to work in an ice/water bath). 

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