Optimising Pepsin Digests

Nigel J.Osborn nigel at njosborn.demon.co.uk
Sat Mar 30 16:22:49 EST 1996

Could anyone provide any information whatsoever as to how one might go
about optimising pepsin digests (to give F(ab')2s) of IgGs. 

What physically happens if you leave the reaction to go too long? 

What causes any loss of antibody activity? 

How one might detect this? Eg isoelectric focussing gels, SDS PAGE etc.

Thanks for any help you can provide.

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