FITC labelled fluorescence

Ross La Motte rlamot1 at
Sat May 4 19:35:45 EST 1996

In article <axc19.3.3187E17D at> Aida Cancel, axc19 at writes:
>I am having troubles detecting an antigen using FITC labelled second

Try using PE-labeled antibodies. I haven't had an experience in tussue
staining, but I've noticed that cells usually have much less
autofluoresence in red spectra and using PE-labeled antibodies with
flow-cytometry I was able to detect cells expressing low levels of an
antigen, while FITC couldn't provide such sensitivity. So it's likely
that PE-antibodies could work better than FITC-Ab in tussiue staining as


More information about the Immuno mailing list